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mouse anti brca1  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse anti brca1
    Mouse Anti Brca1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 727 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+brca1+antibody/BRCA1/pm41922714-203-24-42
    Average 96 stars, based on 727 article reviews
    mouse anti brca1 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: Mechanism of BRCA1-BARD1 function in DNA end resection and DNA protection.
    Article Snippet: Beads were again washed three times with 150 μl of wash buffer (25 mM Tris-HCl pH 7.5, 1 mM DTT, 3 mM EDTA, 80 mM NaCl, 0.05% Triton-X) and proteins were eluted by boiling the beads in SDS buffer (50 mM Tris-HCl pH 6.8, 1.6% SDS, 100 mM DTT, 10% glycerol, 0.01% bromophenol blue) at 95 °C for 3 min. Avidin (Sigma) was added to the eluate as a stabilizer. .. The eluate was separated on a 7.5% SDS–PAGE gel and proteins were detected by western blotting using anti-BRCA1 antibody (Santa Cruz Biotechnology, sc-6954, 1:1,000), anti-His antibody (Invitrogen PA1-983B, 1:1,000) or anti-FLAG antibody (Sigma, F3165, 1:1,000). .. The final images were acquired with Fusion FX7 capture software (Vilber Imaging).

    Article Title: Mechanism of BRCA1–BARD1 function in DNA end resection and DNA protection
    Article Snippet: Beads were again washed three times with 150 μl of wash buffer (25 mM Tris-HCl pH 7.5, 1 mM DTT, 3 mM EDTA, 80 mM NaCl, 0.05% Triton-X) and proteins were eluted by boiling the beads in SDS buffer (50 mM Tris-HCl pH 6.8, 1.6% SDS, 100 mM DTT, 10% glycerol, 0.01% bromophenol blue) at 95 °C for 3 min. Avidin (Sigma) was added to the eluate as a stabilizer. .. The eluate was separated on a 7.5% SDS–PAGE gel and proteins were detected by western blotting using anti-BRCA1 antibody (Santa Cruz Biotechnology, sc-6954, 1:1,000), anti-His antibody (Invitrogen PA1-983B, 1:1,000) or anti-FLAG antibody (Sigma, F3165, 1:1,000). .. The final images were acquired with Fusion FX7 capture software (Vilber Imaging).

    Chromatin Immunoprecipitation:

    Article Title: Targeting branched N -glycans and fucosylation sensitizes ovarian tumors to immune checkpoint blockade
    Article Snippet: Chromatin was digested with micrococcal nuclease (MNase Cell Signaling, Cat#: 10010) in digestion buffer (10 mM Tris pH 8.0, 1 mM CaCl2, and 0.2% Triton X-100) at 37 °C for 15 min. Nucleus products were broken down by Bioruptor pulse at high frequency. .. The following antibodies were used for ChIP: anti-BRCA1 antibody (Santa Cruz, sc-6954, RRID:AB_626761; 5 μg/IP) and anti-BRCA2 antibody (Cell Signaling, 10741, RRID:AB_2797730; 10 μL/IP). .. ChIP DNA was purified by the ChIP DNA clean and concentrator kit (Zymo Research, Cat#: D5205) and analyzed by qPCR.

    Magnetic Beads:

    Article Title: Mechanism of BRCA1-BARD1 function in DNA end resection and DNA protection.
    Article Snippet: Signals were quantified using ImageJ2 (NIH) and plotted with Prism 10 (GraphPad). .. To test the interaction between BRCA1–BARD1 and WRN or EXO1, 1 μg of anti-BRCA1 antibody (Santa Cruz Biotechnology, sc-6954) or anti-WRN antibody (Cell Signaling, 4666S) were captured on 10 μl Protein G magnetic beads (Dynabeads, Invitrogen) by incubating at 4 °C for 1 h with gentle rotation in 50 μl of PBS-T (PBS with 0.1% Tween-20, Sigma). ..

    Article Title: Mechanism of BRCA1–BARD1 function in DNA end resection and DNA protection
    Article Snippet: Signals were quantified using ImageJ2 (NIH) and plotted with Prism 10 (GraphPad). .. To test the interaction between BRCA1–BARD1 and WRN or EXO1, 1 μg of anti-BRCA1 antibody (Santa Cruz Biotechnology, sc-6954) or anti-WRN antibody (Cell Signaling, 4666S) were captured on 10 μl Protein G magnetic beads (Dynabeads, Invitrogen) by incubating at 4 °C for 1 h with gentle rotation in 50 μl of PBS-T (PBS with 0.1% Tween-20, Sigma). ..

    Gentle:

    Article Title: Mechanism of BRCA1-BARD1 function in DNA end resection and DNA protection.
    Article Snippet: Signals were quantified using ImageJ2 (NIH) and plotted with Prism 10 (GraphPad). .. To test the interaction between BRCA1–BARD1 and WRN or EXO1, 1 μg of anti-BRCA1 antibody (Santa Cruz Biotechnology, sc-6954) or anti-WRN antibody (Cell Signaling, 4666S) were captured on 10 μl Protein G magnetic beads (Dynabeads, Invitrogen) by incubating at 4 °C for 1 h with gentle rotation in 50 μl of PBS-T (PBS with 0.1% Tween-20, Sigma). ..

    Article Title: Mechanism of BRCA1–BARD1 function in DNA end resection and DNA protection
    Article Snippet: Signals were quantified using ImageJ2 (NIH) and plotted with Prism 10 (GraphPad). .. To test the interaction between BRCA1–BARD1 and WRN or EXO1, 1 μg of anti-BRCA1 antibody (Santa Cruz Biotechnology, sc-6954) or anti-WRN antibody (Cell Signaling, 4666S) were captured on 10 μl Protein G magnetic beads (Dynabeads, Invitrogen) by incubating at 4 °C for 1 h with gentle rotation in 50 μl of PBS-T (PBS with 0.1% Tween-20, Sigma). ..

    Incubation:

    Article Title: The deubiquitinating enzyme USP4 regulates BRCA1 stability and function
    Article Snippet: .. The lysates were incubated with anti-BRCA1 antibody (Santa Cruz, sc-6954) for 3 h, followed by incubation with protein-A/G agarose for additional 8 h at 4 °C. ..

    Article Title: The deubiquitinating enzyme USP4 regulates BRCA1 stability and function
    Article Snippet: .. The lysates were incubated with anti-BRCA1 antibody (Santa Cruz, sc-6954) for 3 h, followed by incubation with protein A/G agarose for additional 8 h at 4°C. ..



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